SubtiBank SubtiBank
Version comparison:

2018-02-13T15:33:14.000Z2025-05-31 20:32:25

locus

BSU37050

BSU_37050

geneLength

1746

1749

outlinks

bsu

BSU37050

BSU_37050

Gene

Coordinates

3,800,418 → 3,802,166

3,800,418 3,802,166

The protein

Catalyzed reaction/ biological activity

(S)-malate + NAD+ = pyruvate + CO2 + NADH (according to Swiss-Prot) malate -→ pyruvate

(S)-malate + NAD+ --> CO2 + NADH + pyruvate (according to UniProt)

H+ + oxaloacetate --> CO2 + pyruvate (according to UniProt)

The protein

Protein family

malic enzymes family (according to Swiss-Prot)

[SW|malic enzymes family] (according to UniProt)

The protein

Paralogous protein(s)

[[protein|MalS]]

[[this]]

Biological materials

Mutant

MGNA-B657 (ywkA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1656 NBRP B. subtilis, Japan]

GP1141 (aphA3) available in [SW|Stülke] lab

BKE37050 (Δ[[gene|maeA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAAAATATGACCGATC, downstream forward: _UP4_TAATTAGCGAGTAAATTTTT

BKK37050 (Δ[[gene|maeA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAAAATATGACCGATC, downstream forward: _UP4_TAATTAGCGAGTAAATTTTT

MGNA-B657 (ywkA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1656 NBRP B. subtilis, Japan]

GP1141 (aphA3) available in [SW|Jörg Stülke]'s lab

BKE37050 ([[gene|maeA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAAAATATGACCGATC, downstream forward: _UP4_TAATTAGCGAGTAAATTTTT

BKK37050 ([[gene|maeA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAAAATATGACCGATC, downstream forward: _UP4_TAATTAGCGAGTAAATTTTT

Labs working on this gene/protein

[SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

References

21815947, 23136871, 12949160, 16788182, 28974613

21815947, 23136871, 12949160, 16788182, 28974613, 12033925

The protein

Structure

[PDB|1LLQ] (from Ascaris suum, 41% identity) [pubmed|12033925]

labs

[SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France