2018-02-13T15:33:14.000Z2025-05-31 20:32:25
locus
BSU37050
BSU_37050
geneLength
1746
1749
outlinks
bsu
BSU37050
BSU_37050
Gene
Coordinates
3,800,418 → 3,802,166
3,800,418 3,802,166
The protein
Catalyzed reaction/ biological activity
(S)-malate + NAD+ = pyruvate + CO2 + NADH (according to Swiss-Prot) malate -→ pyruvate
(S)-malate + NAD+ --> CO2 + NADH + pyruvate (according to UniProt)
H+ + oxaloacetate --> CO2 + pyruvate (according to UniProt)
The protein
Protein family
malic enzymes family (according to Swiss-Prot)
[SW|malic enzymes family] (according to UniProt)
The protein
Paralogous protein(s)
[[protein|MalS]]
[[this]]
Biological materials
Mutant
MGNA-B657 (ywkA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1656 NBRP B. subtilis, Japan]
GP1141 (aphA3) available in [SW|Stülke] lab
BKE37050 (Δ[[gene|maeA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAAAATATGACCGATC, downstream forward: _UP4_TAATTAGCGAGTAAATTTTT
BKK37050 (Δ[[gene|maeA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAAAATATGACCGATC, downstream forward: _UP4_TAATTAGCGAGTAAATTTTT
MGNA-B657 (ywkA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1656 NBRP B. subtilis, Japan]
GP1141 (aphA3) available in [SW|Jörg Stülke]'s lab
BKE37050 ([[gene|maeA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAAAATATGACCGATC, downstream forward: _UP4_TAATTAGCGAGTAAATTTTT
BKK37050 ([[gene|maeA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAAAATATGACCGATC, downstream forward: _UP4_TAATTAGCGAGTAAATTTTT
Labs working on this gene/protein
[SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
References
The protein
Structure
[PDB|1LLQ] (from Ascaris suum, 41% identity) [pubmed|12033925]
labs
[SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France